Journal: Cell reports
Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy
doi: 10.1016/j.celrep.2020.108434
Figure Lengend Snippet: (A) Baseline number of foci per nucleus as a function of age in CD3 + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.
Article Snippet: EasySep CD3+ Whole Blood Selection Kit , StemCell Technologies , Cat# 18081.
Techniques: Extraction, Cell Culture